Pharm-Rx Methotrexate USP

Chemical Name: Methotrexate

CAS Number: 59-05-2

Certifications & Compliance: United States Pharmacopeia (USP)

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    Enhanced TDS

    Identification & Functionality

    Chemical Name
    Molecular formula
    Product Families
    Chemical Structure

    Pharm-Rx Methotrexate USP - Chemical Structure - 1


    Methotrexate contains NLT 98.0% and NMT 102.0% of methotrexate (C₂₀H₂₂N₈O₅), calculated on the anhydrous basis.

    Applications & Uses


    Typical Properties
    ValueUnitsTest Method / Conditions
    Molecular Weight454.45--

    Regulatory & Compliance

    Certifications & Compliance
    FDA Disclaimer

    These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

    USP Reference Standards
    • USP R-Methotrexate RS
    • (R)-2-(4-{[(2,4-Diaminopteridin-6-yl)methyl](methyl) amino}benzamido)pentanedioic acid.
    • C₂₀H₂₂N₈O₅ 454.45
    • Sodium (R)-2-(4-{[(2,4-Diaminopteridin-6-yl)methyl] (methyl)amino}benzamido)pentanedioate.
    • C₂₀H₂₀N₈Na₂O₅ 498.41
    • USP Methotrexate System Suitability Mixture RS

    This mixture contains:

    • Methotrexate Methotrexate dimethylester hydrochloride; (S)-Dimethyl-2-(4-{[(2,4-diaminopteridin-6-yl)methyl] (methyl)amino}benzamido) pentanedioate hydrochloride. C₂₂H₂₆N₈O₅
    • Methotrexate related compound I; (S)-4-(4-{[(2,4-Diaminopteridin-6-yl)methyl](methyl) amino}benzamido)-5-methoxy-5-oxopentanoic acid. C₂₁H₂₄N₈O₅ 468.47
    • USP Methotrexate RS USP Methotrexate Related Compound B RS (S)-2-{4-[(2,4-Diaminopteridin-6-yl)methylamino] benzamido}pentanedioic acid. C₁₉H₂₀N₈O₅ 440.42 USP
    • Methotrexate Related Compound C RS (S)-2-(4-{[(2-Amino-4-oxo-1,4-dihydropteridin-6-yl) methyl](methyl)amino}benzamido)pentanedioic acid. C₂₀H₂₁N₇O₆ 455.42
    • USP Methotrexate Related Compound E RS 4-{[(2,4-Diaminopteridin-6-yl)methyl](methyl)amino} benzoic acid, hemihydrochloride. C₁₅H₁₅N₇O₂ · 1⁄2HCl 343.56

    Storage & Handling

    Storage Information

    Preserve in tight, light-resistant containers.